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KMID : 0379119850130020083
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Korean Journal of Mycology
1985 Volume.13 No. 2 p.83 ~ p.87
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Steroid Modification with Aspergillus phoenicis
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Abstract
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The temperature dependency and the stability of enzyme systems for 11α-hydroxylation of progesterone were investigated using Aspergillus phoenicis. Though A. phoenicis conserves high enzyme activities for lactose hydrolysis even at high temperatures, the bioconversion reaction of progesterone by this strain was found to be very temperature sensitive. The compositions of reaction mediums of inside and outside of cells were analyzed using sonication technique. At early stage of reaction, the concentration of 11α-hydroxyprogesterone of cell inside was higher than that of outside. But as the reaction proceeded further, the 11α-hydroxyprogesterone existing inside of cells being converted into another products, its concentration was lower within the cells that in the bulk medium. Even in the reaction mediums containing organic solvents, A. phoenicis was founded to be able to metabolite, so that 11α-hydroxyprogesterone can be produced continuously from fixed bed reactions packed with immobilized A phoenicis in vivo.
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